ABSTRACT
Objective@#To explore the relationship between abuse experience with suicidal ideation and suicide attempts of junior middle school students, and to provide a reference for suicide prevention of junior middle school students.@*Methods@#Cluster sampling method were used to selct 10 289 junior middle school students from 25 districts and counties of Chongqing were included in the analysis of this study from July to September in 2020. And Questionnaire on Abuse in Childhood and Mental Health Scale for Middle School Students were applied to collect the data about demographic information, suicide ideation and behavior.@*Results@#The prevalence of suicidal ideation and suicide attempts among junior middle school students were 20.93% and 10.83%, respectively. Multivariate Logistic regression model found that after controlling for demographic variables and mental health, emotional abuse ( OR =2.07) and emotional neglect ( OR =2.03) showed higher correlations with suicidal ideation than the other three types of childhood abuse( OR physical neglect =1.19, OR physical abuse =1.60, OR sexual abuse =1.37, P <0.05); and sexual abuse ( OR =2.29) and physical neglect ( OR =1.87) showed higher associations with suicide attempt than the other three types of abuse( OR emotional abuse =1.63, OR emotional neglect =1.59, OR physical abuse =1.50, P <0.01).@*Conclusion@#All five types of child abuse were independent risk factors for suicidal ideation and suicide attempts, and emotional neglect and emotional abuse had a greater effect on suicidal ideation, sexual abuse and physical neglect had a greater effect on suicide attempts.
ABSTRACT
Background To explore the pharmacodynamic evaluation and mechanism research of BOC26P against breastcancer, and to provide a basis for the treatment of breast cancer.Method MTT assay was used to detect the cytotoxicity of BOC26P against 4 breast cancer cell lines (MCF7/TAX, MDA-MB-231/PT, MDA-MB-231and MCF-7), and as well as the non-tumor cell lines MCF-10A, in variousdrug concentrations (from 0.004 to 1 μM). Western Blotting and Real-Time PCR assay were used to detect therelative protein and gene expression level after treatment with BOC26P in MCF-7/TAX. The effect of BOC26Pon Specific fluorescent P-gp substrate accumulation in MCF-7/TAX was analyzed by flow cytometry; Moleculardocking was used to analyze the binding capacity between BOC26P, Cyclosporine A, and Verapamil. FCM assaystaining with Annexin V-FITC/PI and Propidium iodide was used to measure the apoptosis and the cell cycleafter treatment with BOC26P in MCF-7/TAX, MDA-MB-231/PT, MDA-MB-231, and MCF-7; Detection ofmitochondrial membrane potential after treatment with BOC26P inMCF-7/TAX, MDA-MB-231/PT, MDA-MB231and MCF-7; Western Blotting and Real-Time PCR assay was used to detect the apoptosis relative proteinand gene expression level after treatment with BOC26P in MDA-MB-231, MCF-7, MDA-MB-231/PT, and MCF7/ADR.Results Cytotoxicity assay showed that BOC26P could effectively suppress 4 breast cancer cell lines (MCF7/TAX, MDA-MB-231/PT, MDA-MB-231, and MCF-7) with an IC50 value of under 0.5 μM. The IC50 value ofBOC26P on non-tumor cells MCF-10A was 32.29 μM. The binding ability of BOC26P to P-gp in breast cancercells was weak. There was no significant effect on the intracellular accumulation of Rhodamin 123(Rh123), Pgp binding specific fluorescence substrate, and multi-drug resistance protein P-gp expression in MCF-7/ADRand MDA-MB-231/PT tumor cells; BOC26P induced MCF-7/TAX, MDA-MB-231/PT, MDA-MB-231 and MCF-7cells cycle arrest at G2/M phase and lead to cell apoptosis. BOC26P induced significant activation of p53protein in MCF-7/ADR and MAD-MB-231/TAX cells. Under the same conditions, BOC26P promoted Baxexpression while inhibited Bcl-2 expression, and could significantly cause activation of Cleveland PARP andClevead Caspase3. The results demonstrated that BOC26P may induce apoptosis through the death receptorapoptosis pathway.Conclusion It is known that BOC26P has a significant proliferation inhibitory effect on breast cancer cellswithout serious side effects. BOC26P has the Potential to be developed into a clinical substitute drug for triple-
ABSTRACT
Background Colorectal cancer (CRC) is the most common malignant tumor of digestive system. The metastasesis the main cause of mortality in CRC patients, of whom the initial diagnosis is about 25%. In our study, weaimed to identify potential gene biomarkers based on RNA sequencing data to predict and improve CRCpatient survival.Method In this study, by screening differentially expressed genes of colon cancer related to liver metastasis, asurvival prognostic risk model was constructed by bioinformatics analysis. Here, we conducted our data mininganalysis for CRC by integrating the differentially expressed genes acquired from Gene Expression Omnibus(GEO) database by primary tumor versus liver metastasis (GSE81582，GSE41258，GSE49355，GSE68468)into The Cancer Genome Atlas (TCGA) database which includes 415 primary tumor and 132 liver metastasistissue. At the same time, we used transwell, RT-PCR and western to examine the effects of CLCA1 and SPINK4on the migration of colorectal cancer cells at the cell level.Results We identified intersections of 197 genes (117 up-regulated and 80 down-regulated) between GEO dataand TCGA data. Differentially expressed genes in TCGA-COAD by single factor cox analysis, lasso cycle trainingand multifactor cox analysis composed a survival prognosis prediction model consisted of 7 genes ORM1,CLCA1, C8B, SPINK4, ALDOB, GAMT, C8G. And results of transwell experiments showed that high expression ofCLCA1 and SPINK4 can inhibit the migration ability of colon cancer cells LOVO and SW620, meanwhile westernblotting showed that the high expression of both genes can upregulate the expression of epithelial phenotypicmarker E-cadherin, and Vimentin expression is down-regulated.Conclusion In this study, 197 differentially expressed genes were selected and a relatively robust survivalprognosis prediction model was constructed. The model consisted of seven genes: GAMT, C8G, ORM1, CLCA1,C8B, SPINK4, and ALDOB. At the same time, we found that CLCA1 and SPINK4 are closely related to survivalprognosis. The predictive model nomogram will enable patients with CRC to be more accurately managed intrials testing new drugs and in clinical practice.